Press Release on Bone Marrow Research: August-2018

Bone marrow transplant cleared two men of HIV

More than 3 years past, Australian researchers at St Vincent’s Hospital and therefore the Kirby Institute at the University of recent South Wales (UNSW) transplanted bone marrow to 2 HIV-positive men. Now, the boy’s ar apparently HIV-free.

The researchers have highlighted the actual fact that the bone marrow used failed to contain each copy of the sequence CCR5 delta32, a mutation that protects against the HIV virus, that recommend that bone marrow while not this sequence might even be wont to afford protection againts the virus. Both men, however, have continuing antiretroviral therapy—a combination of medication that stops the progression of the disease—as a protecting life. [1]

Cajanine promotes osteogenic differentiation and proliferation of human bone marrow mesenchymal stem cells.

Seed cells – mesenchymal stem cells (MSCs) – seem to be a horny tool within the context of tissue engineering. Bone marrow represents the most supply of MSCs for each experimental and clinical studies. However, the quantity limitation of bone marrow MSCs (BMSCs) and attenuated perform caused by proliferation create the explore for adequate various sources of those cells for autologous and allogeneic transplant necessary. This study was aimed to analyze the roles of cajanine isolated from the extracts of pigeon pea L. Millsp. within the proliferation and differentiation of BMSCs, and to get the mechanism of the proliferation of BMSCs promoted by cajanine.Bone marrow mesenchymal stem cells were civilized in high-glucose Dulbecco’s changed Eagle’s Medium (DMEM) and osteogenic differentiation was evoked by adding Hexadrol, ascorbic acid and β-glycerophosphate supplements. Bone marrow MSCs were civilized in the medium while not cajanine or supplemented with cajanine. the data concerning the proliferation and osteogenic differentiation of BMSCs was collated. The osteogenic differentiation potential of BMSCs was additionally assessed at the third passage by Von Kossa staining. [2]

Effects of the Use of a Mallet during Bone Marrow Biopsy Collection on Specimen Quality: A Quality Improvement Project

Context: Avoiding procedure-related morphologic distortion equivalent to fragmentation and crush physical object is crucial in bone marrow identification. Use of a hammer or mallet, though infrequent, could be a noted technique of advancing the diagnostic assay needle throughout specimen assortment.

Objectives: we tend to perform a double-blinded, retrospective review of bone marrow biopsies collected by the Interventional Radiology department at our establishment so as to assess specimen quality by victimization this system. [3]

Short lifespans of memory T-cells in bone marrow, blood and lymph nodes suggest that T-cell memory is maintained by continuous self-renewal of recirculating cells

Memory T-cells are essential to keep up semipermanent medicine memory. it’s widely accepted that the bone marrow (BM) plays an important role within the semipermanent maintenance of memory T-cells. there’s arguing but on the longevity and recirculating mechanics of BM memory T-cells. whereas some have projected that the BM could be a reservoir for long-lasting, non-circulating memory T-cells, it’s conjointly been urged to be the advantageous website for memory T-cell self-renewal. during this study, we have a tendency to employed in vivo isotope labeling in goats to at the same time quantify the common turnover rates – and thereby expected lifespans – of memory T-cells from BM, blood, and body fluid nodes (LN). [4]

Bone Marrow Mast Cell Density Correlates with Circulating Biomarkers of Bone Disease in Multiple Myeloma

Aims: there’s inflated vegetative cell density (MCD) in myeloma (MM) bone marrow (BM) that correlate with advanced malady stage. Mast cells (MCs) manufacture varied mediators promoting millimeter growth and bone metabolism. The aim of this study was to gauge whether or not BM MCD correlates with markers of bone metabolism, equivalent to current levels of osteoprotegerin (OPG), soluble receptor activator of nuclear factor-κB ligand (sRANKL), osteopontin (OPN) and phagocyte inflammatory protein-1 alpha (MIP-1 alpha).

Study Design: this can be a cross-sectional study.

Place and period of Study: Department of drugs, University of Kriti, Department, and Laboratory of medical specialty, University Hospital of Heraklion, Department of medical specialty and general medicine, Venizelion Hospital of Heraklion, Department of medical specialty, General Hospital of Chania, between Jan 2010 and December 2014. [5]

Reference

[1] Bone marrow transplant cleared two men of HIV

SCIENCEALERT STAFF    21 JUL 2014 (web link)

[2] Cajanine promotes osteogenic differentiation and proliferation of human bone marrow mesenchymal stem cells.

Zhao ZY, Yang L, Mu X, Xu L, Yu X, Jiao Y, Zhang X, Fu L. Cajanine promotes osteogenic differentiation and proliferation of human bone marrow mesenchymal stem cells. Advances in clinical and experimental medicine: official organ Wroclaw Medical University. 2018 Aug. (Web link)

[3] Effects of the Use of a Mallet during Bone Marrow Biopsy Collection on Specimen Quality: A Quality Improvement Project

Mauzo SH, Golardi N, Baxter AJ, Sultana S, Nguyen ND, Zhang R, Kott MM, Zvavanjanja RC, Chen L. Effects of the Use of a Mallet during Bone Marrow Biopsy Collection on Specimen Quality: A Quality Improvement Project. Annals of Clinical & Laboratory Science. 2018 Jul 1;48(4):517-21. (web link)

[4] Short lifespans of memory T-cells in bone marrow, blood and lymph nodes suggest that T-cell memory is maintained by continuous self-renewal of recirculating cells

Baliu-Piqué M, Verheij M, Drylewicz J, Ravesloot L, De Boer RJ, Koets A, Tesselaar K, Borghans J. Short lifespans of memory T-cells in bone marrow, blood and lymph nodes suggest that T-cell memory is maintained by continuous self-renewal of recirculating cells. Frontiers in Immunology. 2018;9:2054. (web link)

[5] Bone Marrow Mast Cell Density Correlates with Circulating Biomarkers of Bone Disease in Multiple Myeloma

Rodanthi Vyzoukaki¹, Maria Devetzoglou², Constantina A. Pappa³, Andreas N. Antonakis⁴, Samer Masud¹, Anastasia Papadopoulou¹, George Tsirakis⁵ and Michael G. Alexandrakis^2*

¹Laboratory of Hematology, University Hospital of Heraklion, Voutes, Heraklion, 71110, Greece.

²Department of Hematology, University Hospital of Heraklion, Voutes, Heraklion, 71110, Greece.

³Department of Internal Medicine, Venizelion Hospital of Heraklion, Knossos Avenue, Heraklion, Greece.

⁴Department of Medicine, University of Crete, Voutes, Heraklion, 71110, Greece.

⁵Department of Hematology, General Hospital of Chania, Chania, Greece.  (web link)