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An Organic Solvent-Free and Space-Saving Method for Quantifying Cyromazine and Its Metabolite, Melamine, in Cow’s Milk

July 27, 2020 by Editor NetKumar

The author described an organic solvent-free, rapid, simple, and space-saving method of sample
preparation followed by HPLC coupled photo-diode array (PDA) detector for simultaneous
quantification of cyromazine (CYR) and its metabolite, melamine (MEL), in milk. The HPLC-PDA was
performed on an Inertsil® HILIC column with an isocratic aqueous mobile phase. Analytes were
extracted from the sample using water, and purified by Mono-Spin®-C18, a centrifugal monolithic silica
spin mini-columns, and quantified within 20 min. The method, performed under 100% aqueous
conditions, obtained average recoveries for CYR and MEL in the range of 93.2%-99.1% with relative
standard deviations ≤ 2.8%. The quantification limits were 8.5 ng/mL for CYR and 10 ng/mL for MEL,
respectively. No organic solvents were used at any stage of the analysis.

Author (s) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.

View Book:-  http://bp.bookpi.org/index.php/bpi/catalog/book/218

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