Background: Under normal circumstances In the life cycle of Spirometra species, cyclops are the first intermediate hosts.
The purpose of this research is to describe how Cyclops were cultivated in a Tanzanian laboratory from an adult female egg sack Cyclops fed live Paramecium and a stock of Cyclops employed in a Spirometra species life cycle experiment.
Paramecium was first cultured for use as a food source for Cyclops. A sample of water was taken from a pond on the campus of Sokoine University. A swarm of Paramecium was created when 100 mL of water and pre-boiled wheat grains were transferred to a Petri dish and incubated under laboratory conditions for 7 days. In a new Petri dish containing tap water, 0.3 cc of Paramecium suspension, and 4 pre-boiled wheat grains, an adult female egg sack Cyclops from a natural water pond in Tarangire National Park, Tanzania was introduced. The mixture was stored at a temperature of 26-29°C in a laboratory and monitored on a daily basis.
Eggs laid by a single Cyclops hatched into nauplius. The average development time from nauplius I to copepodite I was 1.2 days, copepodite I to adult female Cyclops was 6.9 days, and copepodite I to adult female Cyclops was 26.3 days. The adult male was 846.3 metres long and 284.6 metres wide, whereas naupliusI was 120.2 metres long and 80.0 metres wide. 1 to 8 broods were generated by the mature female (mean 4.3). Cyclops lived for an average of 43.1 days.
Conclusion: In the experimental life cycle of Tanzanian Spirometra species, laboratory-cultured Cyclops were fed Paramecium and served as the first intermediate host.
Nicholas Jairo Kavana
St. Francis University College of Health and Allied Sciences, P.O Box 175, Ifakara, Tanzania.
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