Bitter taste receptors are expressed in human epithelial ovarian and prostate cancers cells and noscapine stimulation impacts cell survival
Bitter style receptors (Tas2Rs) are a taxonomic group of G-protein coupled receptors expressed not solely within the rima however additionally in many extra-oral tissues and sickness states. many natural bitter compounds from plants, like bitter melon extract and noscapine, have displayed anti-cancer effects against numerous cancer sorts. during this study, we have a tendency to examined the prevalence of Tas2R subtype expression in many animal tissue gonad or prostatic adenocarcinoma cell lines, and therefore the practicality of Tas2R14 resolve. qPCR analysis of 5 TAS2Rs incontestable that informational RNA expression usually varies greatly in cancer cells compared to traditional tissue. victimization receptor-specific siRNAs, we have a tendency to additionally incontestable that noscapine stimulation of gonad cancer cells enlarged necrobiosis in ovarian cancer cells in a very receptor-dependent, however ROS-independent manner. This study furthers our understanding of the operate of Tas2Rs in gonad cancer by demonstrating that their activation has a sway on cell survival. [1]
Pineapple extract as an efficient anticancer agent in treating human cancer cells
Various studies have planned the malignant tumor effects of pineapple extracts as a mixture therapy in human cancer cells incidental with irradiation. However, more investigations are required to demonstrate the potentials of pineapple extract together irradiation on varied human cancers. [2]
Long non-coding RNA CASC2 regulates Sprouty2 via functioning as a competing endogenous RNA for miR-183 to modulate the sensitivity of prostate cancer cells to docetaxel
Prostate cancer (PC) is that the commonest cancer in men; but, restricted result is obtained because of the medical aid resistance. CASC2 acts as a tumour suppressor in human malignancies serving as a ceRNA for miRNAs; Sprouty2 (SPRY2), a key antagonist of RTK communication, conjointly is a tumour suppressor. Herein, CASC2 and SPRY2 expression was down-regulated in computer tissues and cell lines; the overexpression of CASC2 and SPRY2 may suppress computer cell proliferation, promote computer cell cell death, and enhance the sensitivity of computer cells to docetaxel. CASC2 absolutely regulated SPRY2 expression and stifled downstream living thing regulated supermolecule kinases (ERK) communication activation through SPRY2. By victimization on-line tools, miR-183 may well be an immediate target of CASC2, and may at the same time bind to the 3′UTR of SPRY2. The direct binding between CASC2, miR-183 and SPRY2 was then validated; miR-183 inhibition increased the toxicity of docetaxel on computer cells, that may be partly attenuated by SPRY2 knockdown. In summary, CASC2 competes with SPRY2 for miR-183 binding to rescue the expression of SPRY2 in computer cells, therefore enhancing the sensitivity of computer cells to docetaxel through SPRY2 downstream ERK communication pathway; CASC2 and SPRY2 may well be novel adjuvants for docetaxel-based therapy for computer. [3]
Extracellular vesicle-packaged HIF-1α-stabilizing lncRNA from tumour-associated macrophages regulates aerobic glycolysis of breast cancer cells
Metabolic reprogramming could be a hallmark of cancer. Here, we tend to demonstrate that tumour-associated macrophages (TAMs) enhance the aerobic metabolism and apoptotic resistance of carcinoma cells via the extracellular sac
(EV) transmission of a myeloid-specific lncRNA, HIF-1α-stabilizing long noncoding ribonucleic acid (HISLA). Mechanistically, HISLA blocks the interaction of PHD2 and HIF-1α to inhibit the hydroxylation and degradation of HIF-1α. Reciprocally, feed discharged from glycolytic tumor cells upregulates HISLA in macrophages, constituting a feed-forward loop between TAMs and tumor cells. obstruction EV-transmitted HISLA inhibits the metabolism and chemoresistance of carcinoma in vivo. Clinically, HISLA expression in TAMs is related to metabolism, poor chemotherapeutical response and shorter survival of patients with carcinoma. Our study highlights the potential of lncRNAs as signal transducers that are transmitted between immune and tumor cells via EVs to push cancer aerobic metabolism. [4]
Retracted: Bioenergetics of Human Cancer Cells and Normal Cells during Proliferation and Differentiation
Cancer cells are legendary to possess completely different metabolic properties than traditional cells, notably their tendency to bear metastasis even beneath aerobic affirmative conditions. This has created interest in however mitochondrial operate in growth cells could take issue from that in traditional cells. victimisation human malignant cells (SW-620, PC-3, HT-1080, SK-MEL, HL-60, K-562 and MOLT-3), human embryonic cell (CCL-153) and human T Cells, we tend to investigated 3 key parameters that are usually to explain mitochondrial function: cellular adenosine triphosphate production, mitochondrial potential and cellular cardiolipin levels. On average, growth cancer cells had additional adenosine triphosphate production and larger mitochondrial potentials. as an example, adenosine triphosphate levels in malignant cells ranged from twenty to sixty nine µmole/106 cells, with a neoplastic cell average of 40±18 µmole/106 cells. For traditional cells, the adenosine triphosphate level vary went from nine to twenty four µmole/106 cells, for a median of 15±11 µmole/106 cells. Mitochondrial potentials attended be thrice higher in cancer cells, maybe as a result of overall mitochondrial mass (as measured by relative cardiolipin levels) were double as high in cancer cells. Higher mitochondrial plenty are in line with proliferation. Proliferating cells normally showed higher mitochondrial operate compared to quiescent cells (confluent monolayers), and HL-60 cells showed reductions altogether 3 mitochondrial parameters measured here once the cells were exposed to the differentiating agent TPA. the consequences of adenosine triphosphate production inhibitors CCCP and oligomycin on mitochondrial operate in traditional and cancer cells were conjointly compared. In general, in these experiments, neoplastic cell mitochondrial inhibition with these agents made a decrease adenosine triphosphate levels by 30-40% whereas in traditional cells ATP production was reduced by sixtieth. These results give proof of a mitochondrial disfunction in cancer cells. Cancer cells seem to higher stand up to interference with adenosine triphosphate synthesis in mitochondria since they trust chiefly on metastasis as associate degree energy manufacturing mechanism.
Retraction Notice: This paper has been backward from British Journal of drugs & Medical analysis thanks to the subsequent reason:
‘The paper was submitted to British Journal of drugs & Medical analysis (BJMMR) on twenty sixth February 2015 and printed in BJMMR on tenth April once critique and editorial acceptance. It came to our information on twenty ninth Gregorian calendar month twenty15 that the identical paper was submitted to the Puerto RICO Health Sciences Journal on October 20, 2014 and authors reciprocally in agreement to retract this paper to avoid copyright connected complexities’. we tend to impart all involved persons for his or her active cooperation, facilitate and patience throughout this method. [5]
Reference
[1] Martin, L.T., Nachtigal, M.W., Selman, T., Nguyen, E., Salsman, J., Dellaire, G. and Dupré, D.J., 2019. Bitter taste receptors are expressed in human epithelial ovarian and prostate cancers cells and noscapine stimulation impacts cell survival. Molecular and Cellular Biochemistry, 454(1-2), pp.203-214. (Web Link)
[2] Raeisi, E., Shahbazi-Gahrouei, D. and Heidarian, E., 2019. Pineapple extract as an efficient anticancer agent in treating human cancer cells. Immunopharmacogenetics, 1. (Web Link)
[3] Gao, W., Lin, S., Cheng, C., Zhu, A., Hu, Y., Shi, Z., Zhang, X. and Hong, Z., 2019. Long non-coding RNA CASC2 regulates Sprouty2 via functioning as a competing endogenous RNA for miR-183 to modulate the sensitivity of prostate cancer cells to docetaxel. Archives of biochemistry and biophysics, 665, pp.69-78. (Web Link)
[4] Extracellular vesicle-packaged HIF-1α-stabilizing lncRNA from tumour-associated macrophages regulates aerobic glycolysis of breast cancer cells
Fei Chen, Jianing Chen, Linbin Yang, Jiang Liu, Xiaoqian Zhang, Yin Zhang, Qingqiang Tu, Dong Yin, Dechen Lin, Ping-Pui Wong, Di Huang, Yue Xing, Jinghua Zhao, Mengfeng Li, Qiang Liu, Fengxi Su, Shicheng Su & Erwei Song
Nature Cell Biologyvolume 21, pages498–510 (2019) (Web Link)
[5] Mikirova, N. A. (2015) “Retracted: Bioenergetics of Human Cancer Cells and Normal Cells during Proliferation and Differentiation”, Journal of Advances in Medicine and Medical Research, 7(12), pp. 971-982. doi: 10.9734/BJMMR/2015/17113. (Web Link)