Bovine in vitro fertilization with frozen-thawed semen
A procedure to get high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed gamete was developed. IVF frequency of in vitro matured oocytes was enhanced by a swimup gamete separation procedure (P=0.01) or treatment of gamete with the glycosaminoglycan anticoagulant (P=0.0001), however the 2 factors didn’t act (P=0.23). anticoagulant was the foremost vital consider increasing IVF frequencies. [1]
Mechanisms of Fertilization in Mammals
The spermatozoa of most invertebrates (e.g., ocean urchins) and nonmammalian vertebrates (e.g., fishes and amphibians) have full capability to fertilize eggs upon departure the sex gland. sex gland spermatozoa of mammals, on the opposite hand, don’t possess the flexibility to try and do thus. Their fertilizing capability develops as they labor under the channel (Young, 1931; Nishikawa and Waide, 1952; Blandau and Rumery, 1964; Bedford, 1966; Orgebin-Crist, 1967). This method, apparently “unique” to mammals, is remarked because the epididymal maturation of spermatozoa. [2]
High fertilization and implantation rates after intracytoplasmic sperm injection
Previously according higher fertilization rate once intra-cytoplasmic single spermatozoon injection (ICSI) than once subzonal insemination of many spermatozoa was confirmed in an exceedingly controlled comparison of the 2 procedures in eleven patients. Intracytoplasmic spermatozoon injection was dispensed in a hundred and fifty consecutive treatment cycles of a hundred and fifty unfertilised couples, World Health Organization had did not have inseminated oocytes once customary in-vitro fertilization (IVF) procedures or World Health Organization weren’t accepted for IVF as a result of not enough motile spermatozoa were gift within the ejaculate. [3]
Micronutrients decline under long-term tillage and nitrogen fertilization
Tillage and atomic number 7 (N) fertilization will be expected to change substance dynamics within the soil and in plants over time. However, quantitative info relating to the consequences of tillage and N application rates on substance dynamics is restricted. The objectives of this study were (a) to work out the long result of various tillage ways similarly as variation in N application rates on the distribution of Mehlich III extractible Mn, copper, zinc, boron, and iron in soils and (b) to assess accumulation of a similar nutrients in wheat (Triticum aestivum L.) tissues. [4]
Foliar Fertilization Using Liquid Tannery Sludge in Conilon Coffee Seedlings Production
The waste destination could be a concern to industries and, one among the solutions are fertilizers. Therefore, the target of this study was to guage the potency liquid work sludge has as an alternate of foliar fertilization in Conilon occasional seedlings. The experiment was disbursed in a very randomised block style with six treatments and eight replications, for 228 days. The management treatment was pure water, and 5 doses of work sludge were applied monthly (6.20, 8.80, 11.47, 14.10, and 17.60 millilitre of sludge diluted in one liter of water). [5]
Reference
[1] Parrish, J.J., Susko-Parrish, J.L., Leibfried-Rutledge, M.L., Critser, E.S., Eyestone, W.H. and First, N.L., 1986. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology, 25(4), pp.591-600. (Web Link)
[2] Yanagimachi, R., 1981. Mechanisms of fertilization in mammals. In Fertilization and embryonic development in vitro (pp. 81-182). Springer, Boston, MA. (Web Link)
[3] Van Steirteghem, A.C., Nagy, Z., Joris, H., Liu, J., Staessen, C., Smitz, J., Wisanto, A. and Devroey, P., 1993. High fertilization and implantation rates after intracytoplasmic sperm injection. Human Reproduction, 8(7), pp.1061-1066. (Web Link)
[4] Micronutrients decline under long-term tillage and nitrogen fertilization
Santosh Shiwakoti, Valtcho D. Zheljazkov, Hero T. Gollany, Markus Kleber & Baoshan Xing
Scientific Reportsvolume 9, Article number: 12020 (2019) (Web Link)
[5] de Sales, R., Rossini, F., Berilli, S. da, Galvão, E., Mendes, T., Gabriel Berilli, A. P., de Salles, R., de Sales, R., Quartezani, W. and Freitas, S. de (2018) “Foliar Fertilization Using Liquid Tannery Sludge in Conilon Coffee Seedlings Production”, Journal of Experimental Agriculture International, 22(2), pp. 1-8. doi: 10.9734/JEAI/2018/40201. (Web Link)