Microbiological analysis of seed sprouts in Norway
As a part of larger survey of microbic contamination of fruits and vegetables in Noreg, four completely different up seed product were analysed for microorganism and parasitic contaminants (n=300 for microorganism analyses and n=from seventeen to 171 for parasite analyses, reckoning on parasite).
Escherichia coli O157, Salmonella, Listeria monocytogenes, Cyclospora oocysts, worm genus eggs and different parasitic worm parasites weren’t detected in any of the sprout samples. Thermotolerant coliform bacterium (TCB) were isolated from some twenty fifth of the sprout samples, with the best share of TCB positive samples in alfalfa sprouts. Most TCB were Enterobacter spp. and enteric bacteria. E. coli was isolated from eight of sixty two TCB positive legume sprout samples. Cryptosporidium oocysts were detected in V-day of the sprout samples and flagellate cysts were detected in a pair of of the samples. All the Cryptosporidium positive samples, and most of the flagellate positive samples, were legume sprouts. Parasite concentrations in positive samples were low (between one and three oocysts/cysts per fifty g sprouts). [1]
Application of FISH technology for microbiological analysis: current state and prospects
In order to spot and quantify the microorganisms gift in an exceedingly bound system, it’s become necessary to develop molecular ways avoiding cultivation, that permits to characterize solely the numerable a part of the microorganisms within the sample, so losing the knowledge associated with the microorganism part that presents a vitality condition, though it cannot duplicate in medium. during this context, one amongst the foremost used techniques is light in place sexual union (FISH) with ribosomal ribonucleic acid targeted oligonucleotide probes. because of its speed and sensitivity, this system is taken into account a robust tool for biological process, ecological, diagnostic and environmental studies in biological science. Through the employment of species-specific probes, it’s attainable to spot totally different microorganisms in complicated microorganism communities, therefore providing a solid support to the understanding of inter-species interaction. [2]
Biotechnical use of polymerase chain reaction for microbiological analysis of biological samples.
Since its introduction within the mid-80s, enzyme chain reaction (PCR) technology has been recognised as a fast, sensitive and specific molecular diagnostic tool for the analysis of micro-organisms in clinical, environmental and food samples. though this system is extraordinarily effective with pure solutions of nucleic acids, it’s sensitivity is also reduced dramatically once applied on to biological samples. This review describes PCR technology as a microbic detection methodology, PCR inhibitors in biological samples and numerous sample preparation techniques that may be accustomed facilitate PCR detection, by either separating the micro-organisms from PCR inhibitors and/or by concentrating the micro-organisms to detectable concentrations. [3]
Calcite moonmilk of microbial origin in the Etruscan Tomba degli Scudi in Tarquinia, Italy
A white deposit covering the walls within the textual matter degli Scudi of the Tomba degli Scudi, Tarquinia, Italy, has been investigated. during this chamber, that remains preserved from any reasonably intervention like cleanup and cleanup, ancient Etruscans painted shields to celebrate the military power of the Velcha family. Scanning microscopy analysis has disclosed the presence of characteristic nanostructures comparable to a spar secondary mineral deposit referred to as moonmilk. [4]
Microbiological Analysis of Dumpsites in Bwari, Federal Capital Territory, Nigeria
Open trash dump system is practiced in Bwari as in most cities in African nation. moldering wastes, soil samples and aerial samples were taken from the 2 major dumpsites in Bwari; Bwari complicated trash dump and Bwari space Council trash dump (referred to dumpsites one and a couple of severally during this article). Sabouraud dextroglucose, MacConkey and culture medium plates in triplicates were exposed at a height of two M round the atmosphere of the dumpsites. management aerial samples were taken regarding two klick removed from the dumpsites. moldering wastes and soil samples were collected from the dumpsites on 10 consecutive days from totally different points of the dumpsites. These samples were civilised and incubated into culture medium and. Potato dextroglucose agar plates severally victimization commonplace ways. [5]
Reference
[1] Robertson, L.J., Johannessen, G.S., Gjerde, B.K. and Loncarevic, S., 2002. Microbiological analysis of seed sprouts in Norway. International Journal of Food Microbiology, 75(1-2), (Web Link)
[2] Bottari, B., Ercolini, D., Gatti, M. and Neviani, E., 2006. Application of FISH technology for microbiological analysis: current state and prospects. Applied Microbiology and Biotechnology, 73(3), (Web Link)
[3] Lantz, P.G., Abu, W.A.S., Knutsson, R., Hahn-Hägerdal, B. and Rådström, P., 2000. Biotechnical use of polymerase chain reaction for microbiological analysis of biological samples. Biotechnology annual review, 5, (Web Link)
[4] Calcite moonmilk of microbial origin in the Etruscan Tomba degli Scudi in Tarquinia, Italy
Angela Cirigliano, Maria Cristina Tomassetti, Marta Di Pietro, Francesco Mura, Maria Lorella Maneschi, Maria Donatella Gentili, Barbara Cardazzo, Chiara Arrighi, Cristina Mazzoni, Rodolfo Negri & Teresa Rinaldi
Scientific Reports volume 8, (Web Link)
[5] Evangeline, U., Oyegue, T. O., Rwang, P. G., Ozoude, T. O. and Okey-Ndeche, N. F. (2017) “Microbiological Analysis of Dumpsites in Bwari, Federal Capital Territory, Nigeria”, Microbiology Research Journal International, 22(3), (Web Link)