A Review on Innovating the Concept and Practice of Two-Dimensional Gel Electrophoresis in the Analysis of Proteomes at the Proteoform Level

A significant and well-established technological platform allowing comprehensive top-down proteomic analysis is the two-dimensional gel electrophoresis (2DE). However, 2DE's long-held but now increasingly obsolete traditional principles have clearly impacted its application at the level of protein species/proteoforms to in-depth investigations

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